Table 2.

Bacterial strains and plasmids

Strain	Relevant Genotype	Source
A. finegoldii	Strain DSM 17242 (DSMZ Collection)	(Rautio et al., 2003)
E. coli SM2-1	plsC101(Ts) metCl6S::Tn10	(Coleman, 1990)
E. coli LCH30	aas-1 fadD88 metB1 relA1 spoT1	(Hsu et al., 1991)
Plasmid	Description	Source
pPJ131	E. coli expression vector	(Lu et al., 2006)
pEcPlsC	E. coli plsC in pPJ131	(Robertson et al., 2017)
pCtPlsC	Chlamydia trachomatis plsC in pPJ131	(Robertson et al., 2017)
pAfPlsC	A. finegoldii plsC (Alfi_2876) in pPJ131	This study
pEcFadD	E. coli fadD in pPJ131	(Yao et al., 2016)
pNgAas	Neisseria gonorrhoeae aas in pPJ131	(Yao et al., 2016)
pAfAas1	A. finegoldii aas1 (Alfi_2635) in pPJ131	This study
pAf3181	A. finegoldii Alfi_3181 in pPJ131	This study
pAfAas2	A. finegoldii aas2 (Alfi_0371) in pPJ131	This study
pET_AfAas1	A. finegoldii aas1 (Alfi_2635) in pET	This study
pET_AfAas2	A. finegoldii aas2 (Alfi_0371) in pET	This study