Fig. 1.

Relative enrichments of CodY-binding regions under different binding conditions. The relative enrichments were compared for two groups of CodY-binding regions comprising the 50 strongest regions (enrichment rankings 1–50) or regions with enrichment rankings from 101 to 200. The maximum enrichment for each region under any of the 24 binding conditions was defined as 100%, and the enrichments under other 23 conditions were normalized to this value. The obtained relative enrichments for individual regions of each group were averaged for each binding condition. The 24 binding assays were performed at CodY concentrations of 0.32, 1.6, 8, 40, 200, and 1,000 nM with four successive cycles of binding (labeled as 1–4) at each concentration.