Table 3.
Expression of lacZ fusions in B. subtilis cells
| Strain | Fusion type | Genotype (codY allele) | Additions to the medium | β-galactosidase activity (Miller units) |
|---|---|---|---|---|
| BB2505 | bcaPbs | codY+bs | none | 53.9 |
| ILV | 7.44 | |||
| 13 aa | 13.6 | |||
| 13 aa + ILV | 0.14 | |||
| BB3781 | codY+lm | none | 33.7 | |
| ILV | 6.90 | |||
| 13 aa | 6.03 | |||
| 13 aa + ILV | 0.13 | |||
| BB2548 | codY::spc | none | 119.0 | |
| 13 aa + ILV | 137.0 | |||
| BB2770 | ybgEbs | codY+bs | none | 19.2 |
| ILV | 3.49 | |||
| 13 aa | 11.7 | |||
| 13 aa + ILV | 1.12 | |||
| BB3782 | codY+lm | none | 14.4 | |
| ILV | 5.92 | |||
| 13 aa | 9.48 | |||
| 13 aa + ILV | 1.00 | |||
| BB2771 | codY::spc | none | 268.0 | |
| 13 aa + ILV | 428.0 | |||
| BB3776 | gdhAlm | codY+bs | none | 676.1 |
| ILV | 660.9 | |||
| 13 aa | 490.1 | |||
| 13 aa + ILV | 236.6 | |||
| BB3784 | codY+lm | none | 655.2 | |
| ILV | 635.1 | |||
| 13 aa | 516.8 | |||
| 13 aa + ILV | 221.8 | |||
| BB3778 | codY::spc | none | 689.3 | |
| 13 aa + ILV | 636.1 |
Cells were grown in TSS glucose-ammonium medium with or without mixtures of ILV or 13 aa or both (see Experimental procedures). β-Galactosidase specific activity was assayed and expressed in Miller units. All values are averages of at least two experiments, and the relative standard errors of the mean did not exceed 20%. Some data for the bcaP283-lacZ and ybgE292-lacZ fusions in a wild-type and codY::spc mutant strains were published previously (Belitsky and Sonenshein, 2011).