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. 2020 Mar 7;118(8):1838–1849. doi: 10.1016/j.bpj.2020.02.027

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(A) Apparent pKa_D97 of WT PR reconstituted in negatively charged POPC/POPG (80/20, mol/mol) liposomes (blue triangle) and positively charged (80/20, mol/mol) liposomes (red triangle) in a 10 mM HEPES buffer. The HEPES buffer contained different concentrations of NaCl between 0 and 150 mM. (B) Apparent pKa_D97 of WT PR reconstituted in negatively charged POPC/POPG (80/20, mol/mol) liposomes in the HEPES buffer containing different concentrations of NaCl (triangle, same as in A), KCl (diamond), CaCl₂ (circle), and MgCl₂ (square) is shown. (C) Intrinsic pKa_D97 of WT PR reconstituted in negatively charged POPC/POPG (80/20, mol/mol) liposomes (blue triangle) and positively charged (80/20, mol/mol) liposomes (red triangle) in a HEPES buffer containing different concentrations of NaCl is shown.