Fig. 2.

CCL2 is a functional important target of the inhibitory effect of AEA. a–c Boyden chamber assay with THP1 leukocytic cells and conditioned medium of HAoSMCs treated with or without 100 nmol/L AEA (2 h) and 10 ng/mL IL-1β (3 h) as indicated (a). Effect of CCL2-neutralizing antibody (b, 1:1000 in condition medium) or CCL2 protein recombinant protein (c, 50 ng/mL in conditioned medium). N = 3–6, ^#p < 0.05—vs. treatment. Ordinary one-way ANOVA with Tukey’s multiple comparison post-hoc test