Table 1.
Step | Recovered parasitic stage | Quantity of parasites after each stepa | Yield (%)a,b |
---|---|---|---|
Cleaning of oocysts with 1-μm filterc | Oocysts | 1.19 ± 0.13 × 105 | 79.44 ± 9.17 |
Oocysts mechanical grind with glass beads | Sporocysts | 3.33 ± 0.55 × 105 | 55.55 ± 9.17 |
Excystation | Sporozoites | 4.46 ± 0.9 × 105 | 39.02 ± 7.50 |
Sporozoites purification with 5-μm filter | Sporozoites | 4.28 ± 1.07 × 105 | 35.69 ± 8.93 |
aQuantities and yields reported are the means ± standard deviations of triplicate
bYield percentages calculated from an initial amount of 1.5 × 105 oocysts
cYield percentages of earlier steps during oocysts cleaning were not calculated