Table 1.

Overall PERMANOVA results using measures of weighted UniFrac distance, unweighted UniFrac distance, and Bray-Curtis dissimilarity to test for main and interaction effects (see Supplemental Table S4 for all ANOVA tables)

	Degrees of freedom	Sum of squares	Mean square	F	p
Weighted UniFrac distance
Population	2	0.193	0.097	4.883	0.001
Temperature	2	0.262	0.131	6.629	0.001
Extraction date	26	1.498	0.058	2.914	0.001
Genotype (nested in population)	6	0.208	0.035	1.752	0.032
Jar (nested in genotype and population)	7	0.200	0.029	1.447	0.107
Population × temperature	3	0.026	0.009	0.441	0.943
Genotype × temperature	8	0.138	0.017	0.874	0.624
Jar × temperature	9	0.184	0.020	1.036	0.449
Residuals	36	0.712	0.020
Unweighted UniFrac distance
Population	2	0.220	0.110	1.101	0.295
Temperature	2	1.019	0.510	5.108	0.001
Extraction date	26	6.227	0.239	2.401	0.001
Genotype (nested in population)	6	0.496	0.083	0.828	0.814
Jar (nested in genotype and population)	7	0.585	0.084	0.837	0.83
Population × temperature	3	0.245	0.082	0.819	0.738
Genotype × temperature	8	0.587	0.073	0.736	0.964
Jar × temperature	9	0.953	0.106	1.061	0.352
Residuals	36	3.591	0.100
Bray-Curtis dissimilarity
Population	2	0.687	0.344	3.028	0.003
Temperature	2	1.313	0.657	5.787	0.001
Extraction date	26	7.963	0.306	2.699	0.001
Genotype (nested in population)	6	0.902	0.150	1.324	0.108
Jar (nested in genotype and population)	7	1.205	0.172	1.517	0.033
Population × temperature	3	0.301	0.100	0.886	0.59
Genotype × temperature	8	0.999	0.125	1.100	0.312
Jar × temperature	9	1.369	0.152	1.341	0.08
Residuals	36	4.085	0.113	0.217

Significant p values after Bonferroni correction are in italics