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. 2020 Apr 22;10:6805. doi: 10.1038/s41598-020-63311-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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smRNA-seq data analysis. (A) Flowchart of the miRNA analysis pipeline. Total RNA prepared from Fig. 3A was subjected to smRNA-seq. Read per millions of mapped reads (RPM) higher than 1 in at least one sample were considered as expressed miRNAs and used for fold-change calculations. Venn diagram showing the miRNAs that are up- or down-regulated more than 1.5-fold by FIR treatment. Colors as described in Fig. 3A. (B) Heatmap of hierarchical cluster analysis of miRNAs simultaneously up- (18) and down-(14) regulated in FIR-responsive male and female CAD ECFCs after FIR treatment. (C) Histogram demonstrating the expression level of the FIR up- (left panel) and down- (right panel) regulated miRNAs in (B).