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. 2020 Apr 22;11:1938. doi: 10.1038/s41467-020-15605-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a, b Bs wt (AB132: lytB-gfp), ∆ymdB (AB134: lytB-gfp, ΔymdB) and ymdB_OE (AB144: lytB-gfp, ΔymdB, amyE::P_hyper-spank-ymdB) strains were spotted onto poly l-lysine coated coverslips, treated with anti-GFP primary antibodies and FITC-conjugated secondary antibodies and visualized by fluorescence microscopy. Shown are phase contrast images (left panels) and respective immunofluorescence images (right panels). Scale bars represent 2 μm (a). Graph presents quantification of the average number of LytB-GFP foci displayed per cell. Shown are mean ± SEM and P values (unpaired Student’s t test) of at least three independent experiments (n_cells = 600) (b). Source data are provided as a Source Data file.