Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 22;11:1938. doi: 10.1038/s41467-020-15605-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — a Bs cells expressing LytB-GFP (AB144: lytB-gfp, ΔymdB, amyE::P_hyper-spank-ymdB, Δhag) were spotted onto EM grids and subjected to immuno-gold XHR-SEM analysis, using primary antibodies against GFP and secondary gold-conjugated antibodies. Samples were not coated before observation. Shown is an overlay of XHR-SEM images acquired using TLD-SE (through lens detector-secondary electron) for nanotube visualization and vCD (low-kV high-contrast detector) for gold particle (white dots) detection (left panel), and the corresponding vCD image (middle panel). Schematic depicts the interpretive cell layout and highlights the region with gold signal captured by XHR-SEM (right panel). b Bs cells expressing LytB-GFP (AB170: lytB-gfp, ΔymdB, amyE::P_hyper-spank-ymdB, Δhag, ∆wapA) were mixed with Bm (OS2, lacking GFP), spotted onto EM grids and subjected to immuno-gold XHR-SEM as described in a. Shown is an overlay of XHR-SEM images acquired using TLD-SE for nanotube visualization and vCD for gold particle (white dots) detection (left panel), and a corresponding vCD image of the boxed region (middle panel). Schematic depicts the interpretive cell layout and highlights the region with gold signal captured by XHR-SEM (right panel). c Bs cells expressing GFP tagged LytB (AB144: lytB-gfp, ΔymdB, amyE::P_hyper-spank-ymdB, Δhag) were mixed with Sa (MRSA, lacking GFP), spotted onto EM grids and subjected to immuno-gold XHR-SEM as described in a. Shown is an overlay of XHR-SEM images acquired using TLD-SE for nanotube visualization and vCD for gold particle (white dots) detection (left panel), and a corresponding vCD image of the boxed region (middle panel). Schematic depicts the interpretive cell layout and highlights the region with gold signal captured by XHR-SEM (right panel). d Bs cells expressing GFP tagged LytB (AB144: lytB-gfp, ΔymdB, amyE::P_hyper-spank-ymdB, Δhag) were mixed with Vc (N16961, lacking GFP), spotted onto EM grids and subjected to immuno-gold XHR-SEM analysis as described in a. Shown is an overlay of XHR-SEM images acquired using TLD-SE for nanotube visualization and vCD for gold particle (white dots) detection (left panel), and the corresponding vCD image (middle panel). Schematic depicts the interpretive cell layout and highlights the region with gold signal captured by XHR-SEM (right panel). Each experiment was repeated at least five times independently with similar results. Scale bars represent 500 nm. Arrows indicate intercellular nanotubes.