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. 2020 Apr 22;10:6826. doi: 10.1038/s41598-020-63815-w

Table 6.

Percent inhibition of enzyme activity in Culex quinquefasciatus larvae using different concentrations of Bti at 30% concentrations.

Plants Concentration AChE AcP AkP α-Carboxyl β-Carboxyl
Z1 50 ppm 14.85 ± 2.83 a 14.90 ± 1.75 a 8.35 ± 1.08 a 30.01 ± 2.02 a 26.65 ± 2.23 a
100 ppm 31.14 ± 3.36 b 21.07 ± 1.43 b 12.95 ± 0.78 a 52.80 ± 3.01 b 43.87 ± 3.34 b
250 ppm 52.94 ± 2.43 c 32.02 ± 1.14 c 21.01 ± 1.57 b 63.88 ± 1.67 c 46.91 ± 3.08 b
F, df and P value (F = 92.99; df = 2; P < 0.05) (F = 32.03; df = 2; P < 0.05) (F = 24.56 df = 2; P < 0.05) (F = 78.92; df = 2; P < 0.05) (F = 23.02; df = 2; P < 0.05)
Z2 50 ppm 17.83 ± 2.3 a 15.77 ± 1.43 a 8.87 ± 1.16 a 24.08 ± 2.13 a 26.13 ± 3.38 a
100 ppm 31.12 ± 3.32 b 20.97 ± 1.03 b 12.91 ± 0.93 b 33.10 ± 4.11 a 39.78 ± 3.03 b
250 ppm 59.95 ± 2.47 c 29.98 ± 1.23 c 26.08 ± 0.69 c 45.04 ± 3.513b 51.01 ± 2.57 b
F, d.f and P value (F = 90.86; df = 2; P < 0.05) (F = 38.79; df = 2; P < 0.05) (F = 128.87; df = 2; P < 0.05) (F = 13.05; df = 2; P < 0.05) (F = 14.03; df = 2; P < 0.05)
Z3 50 ppm 11.93 ± 2.69 a 19.61 ± 1.38 a 9.49 ± 1.19 a 22.93 ± 2.82 a 16.18 ± 3.74 a
100 ppm 24.03 ± 2.59 b 21.83 ± 1.68 a 16.09 ± 1.39 b 36.15 ± 3.08 b 36.24 ± 3.49 b
250 ppm 48.78 ± 1.82 c 28.16 ± 1.24 b 25.02 ± 1.29 c 47.13 ± 3.39 c 47.04 ± 2.52 b
F, d.f and P value (F = 59.57; df = 2; P < 0.05) (F = 12.89; df = 2; P < 0.05) (F = 39.94; df = 2; P < 0.05) (F = 23.29; df = 2; P < 0.05) (F = 23.31; df = 2; P < 0.05)
Z4 50 ppm 20.67 ± 2.87 a 19.96 ± 1.49 a 11.03 ± 1.49 a 28.49 ± 2.59 a 22.03 ± 4.87 a
100 ppm 27.79 ± 2.39 a 23.21 ± 1.54 b 13.48 ± 1.58 a 45.08 ± 2.78 b 35.03 ± 3.61 ab
250 ppm 51.14 ± 2.78 b 29.08 ± 1.29 c 24.98 ± 1.38 b 47.68 ± 3.71 b 49.69 ± 3.89 b
F, d.f and P value (F = 45.89; df = 2; P < 0.05) (F = 42.39; df = 2; P < 0.05) (F = 33.54; df = 2; P < 0.05) (F = 12.89; df = 2; P < 0.05) (F = 11.23; df = 2; P < 0.05)
Z5 50 ppm 20.69 ± 2.03 a 18.07 ± 1.08 a 10.17 ± 0.74 a 37.93 ± 2.11 a 31.01 ± 2.93 a
100 ppm 38.76 ± 3.06 b 23.29 ± 1.39 b 15.34 ± 0.74 b 58.17 ± 2.89 b 48.16 ± 3.04 b
250 ppm 54.68 ± 1.35 c 34.28 ± 1.13 c 24.58 ± 1.14 c 71.08 ± 3.03 c 59.96 ± 1.68 c
F, d.f and P value (F = 59.24; df = 2; P < 0.05) (F = 47.87; df = 2; P < 0.05) (F = 51.02; df = 2; P < 0.05) (F = 52.23; df = 2; P < 0.05) (F = 31.07; df = 2; P < 0.05)

Convulvulus arvensis (Z1), Chenopodium murale (Z2), Tribulus terrestris (Z3), Trianthema portulacastrum (Z4) and Achyranthes aspera (Z5).

AChE = acetylcholinesterase, AcP = acid phosphatases, AkP = alkaline phosphatases, α-Carboxyl = α-Carboxylesterases and β-Carboxyl = β-Carboxylesterases.

Means sharing the same letter within each treatment is not statistically different.