Skip to main content
. 2020 Apr 22;11:1936. doi: 10.1038/s41467-020-15714-x

Fig. 3. Lgr5 expression at the villus tip of Lgr5-knock-in mouse models.

Fig. 3

a GFP fluorescence (green) observed in both crypt base columnar cells (CBCs) and VTTs in Lgr5–GFP-DTR mice. Scale bar–100 µm. b blow up of villus tip with GFP+ VTTs, scale bar–10 µm. c EGFP fluorescence of crypt base columnar cells in Lgr5-EGFP-IRES-creERT2 mice. Scale bar–10 µm. d EGFP fluorescence in VTTs. The Lgr5-EGFP knock-in construct is expressed in a patchy manner in the villus tip stroma. Scale bar–20 µm. Blow up shows Lgr5 mRNAs in red, EGFP mRNA in green and DAPI in blue. Scale bar–2 µm. Yellow blobs are autofluorescent elements.