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. 2020 Apr 22;11:1937. doi: 10.1038/s41467-020-15704-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Construct for single enzyme expression, b construct for two genes which paired α-Xylp_1211 with another enzyme, and c construct for expression of four genes together; three such constructs, as shown in Supplementary Table 11, were bulit. CBP promoter: strong promoter from C. thermocellum that drives the cbp (Clo1313_1954) gene; Kan: Kanamycin resistance marker; E. coli/T. sacch replicon: contains origins of replication for E. coli and T. thermosaccharolyticum/T. saccharolyticum.