. 2020 Apr 22;10:6834. doi: 10.1038/s41598-020-63905-9

Table 3.

Deployment of the interaction between gliricidia levels and opening times for fermentation characteristics.

Item	Opening	Gliricidia levels					SEM
Item	Opening	0	25	50	75	100	SEM
pH	1	4.29 ± 0.02^a	4.46 ± 0.08^a	4.50 ± 0.06^a	4.69 ± 0.06^a	6.17 ± 0.07^a	0.186
	7	3.70 ± 0.02^c	3.86 ± 0.01^b	3.96 ± 0.02^b	4.09 ± 0.04^bc	5.52 ± 0.19^c	0.076
	15	3.90 ± 0.09^b	3.96 ± 0.02^b	4.09 ± 0.02^b	4.28 ± 0.05^b	5.85 ± 0.21^b	0.197
	30	3.80 ± 0.02^bc	3.93 ± 0.08^b	3.98 ± 0.04^b	4.23 ± 0.08^ab	5.38 ± 0.11^d	0.155
	60	3.78 ± 0.05^bc	3.93 ± 0.05^b	4.06 ± 0.02^b	4.18 ± 0.03^abc	5.16 ± 0.16^e	0.132
	90	3.68 ± 0.04^c	3.92 ± 0.06^b	3.94 ± 0.04^b	4.02 ± 0.03^c	4.96 ± 0.07^e	0.119
Buffer capacity (BC)	1	13.75 ± 0.85^b	14.73 ± 0.79^d	17.75 ± 1.74^d	21.71 ± 0.20^c	23.74 ± 2.31^c	0.802
	7	18.55 ± 1.35^a	20.75 ± 0.85^c	24.89 ± 2.00^c	32.00 ± 1.73^b	34.52 ± 1.34^a	1.270
	15	14.20 ± 0.52^ab	26.44 ± 1.39^b	28.45 ± 0.35^ab	30.80 ± 1.24^b	32.49 ± 2.09^b	1.624
	30	16.34 ± 0.22^ab	27.94 ± 0.49^ab	25.85 ± 2.66 ^bc	33.73 ± 1.13^ab	33.41 ± 0.90^ab	1.751
	60	16.42 ± 0.76^ab	28.64 ± 1.26^ab	30.11 ± 0.10^ab	32.45 ± 1.39^ab	34.16 ± 2.92^a	2.429
	90	16.16 ± 0.02^ab	31.69 ± 1.15^a	31.10 ± 0.08^a	36.71 ± 0.18^a	35.41 ± 0.86^a	1.968
Ammoniacal nitrogen (N-NH₃)	1	0.84 ± 0.10^c	3.77 ± 0.48^a	0.83 ± 0.13^c	2.50 ± 0.15^c	3.31 ± 0.16^ab	0.612
	7	5.89 ± 0.68^a	3.80 ± 0.60^a	2.76 ±± 0.18^b	2.84 ± 0.37^bc	4.06 ± 0.43^a	0.566
	15	1.40 ± 0.41^c	2.37 ± 0.11^b	2.51 ± 0.07^b	2.50 ± 0.06^c	1.82 ± 0.09^c	0.220
	30	0.43 ± 0.07^c	2.00 ± 0.10^b	3.05 ± 0.17^b	2.87 ± 0.07^bc	2.97 ± 0.10^abc	0.496
	60	2.96 ± 0.11^b	2.47 ± 0.43^ab	3.09 ± 0.38^b	4.10 ± 0.37^ab	2.98 ± 0.40^ab	0.267
	90	1.01 ± 0.02^c	3.26 ± 0.45^ab	8.16 ±± 0.85^a	4.87 ± 0.48^a	2.30 ± 0.27^bc	1.233
Water-soluble carbohydrates (WSC)	1	15.99 ± 0.75^b	13.67 ± 0.99^a	12.21 ± 1.23^a	10.48 ± 0.71^a	8.46 ± 0.59^a	0.157
	7	19.84 ± 1.79^a	11.59 ± 1.07^b	11.55 ± 0.85^b	6.47 ± 0.39^ab	8.04 ± 0.54^a	2.067
	15	11.64 ± 0.89^b	10.46 ± 0.69^bc	11.61 ± 0.89^b	3.85 ± 0.25^b	4.05 ± 0.29^b	1.608
	30	8.75 ± 1.09^bc	7.44 ± 0.39 ^cd	10.77 ± 0.79^b	8.05 ± 0.55^a	5.58 ± 0.37^ab	0.757
	90	6.11 ± 0.59^c	5.53 ± 0.14^d	5.53 ± 0.49^c	4.84 ± 0.34^ab	3.96 ± 0.19^b	0.329

SEM = standard error of the mean; %TN, Total nitrogen. Averages followed by different letters on the column do not differ among themselves by Tukey’s test (p < 0.05).