. 2020 Apr 22;10:6834. doi: 10.1038/s41598-020-63905-9

Table 5.

Deployment of the interaction between gliricidia levels and opening times for organic acids content.

Item (% DM)	Opening	Gliricidia levels					SEM
Item (% DM)	Opening	0	25	50	75	100	SEM
Lactic acid (LA)	1	2.05 ± 0.39^d	2.87 ± 0.38^b	3.16 ± 0.53^c	2.71 ± 0.37^b	1.36 ± 0.13^b	0.192
	7	4.94 ± 0.97^c	4.51 ± 0.76^b	5.58 ± 1.17^b	7.05 ± 0.30^a	5.43 ± 1.16^a	0.307
	30	6.91 ± 1.30^b	8.95 ± 0.75^a	6.15 ± 0.28^b	6.53 ± 0.07^a	1.84 ± 0.26^b	0.685
	60	9.03 ± 0.82^a	7.22 ± 0.72^a	6.31 ± 0.29^b	5.26 ± 0.36^a	1.67 ± 0.21^b	0.665
	90	6.05 ± 0.58^bc	8.27 ± 0.83^a	8.82 ± 0.30^a	6.06 ± 0.27^a	3.06 ± 0.39^b	0.559
Acetic acid (AA)	1	0.66 ± 0.04^c	0.81 ± 0.17^b	0.65 ± 0.10^c	0.75 ± 0.02^c	0.28 ± 0.02^b	0.055
	7	0.68 ± 0.05^c	0.80 ± 0.07^{a b}	1.03 ± 0.18^bc	1.01 ± 0.19^c	0.69 ± 0.09^b	0.056
	30	1.64 ± 0.07^ab	2.42 ± 0.03^a	1.85 ± 0.23^a	1.36 ± 0.03^bc	1.61 ± 0.14^a	0.159
	60	1.78 ± 0.18^a	1.97 ± 0.16^a	1.31 ± 0.10^abc	1.24 ± 0.06^bc	1.42 ± 0.05^a	0.087
	90	0.97 ± 0.07^bc	2.20 ± 0.17^a	1.69 ± 0.09^ab	1.75 ± 0.08^a	2.05 ± 0.06^a	0.122
Ratio of lactic acid and acetic acid (LA:AA)	1	3.11 ± 0.04^b	3.54 ± 0.20^a	4.86 ± 0.16^a	3.61 ± 0.30^b	4.86 ± 0.02^b	0.221
	7	7.26 ± 0.06^a	5.64 ± 0.17^a	5.42 ± 0.05^a	6.98 ± 0.17^a	7.87 ± 0.36^a	0.496
	30	4.22 ± 0.20^ab	3.70 ± 0.20^a	3.32 ± 0.02^a	4.80 ± 0.13^ab	1.14 ± 0.05^c	0.475
	60	5.07 ± 0.15^ab	3.66 ± 0.16^a	4.82 ± 0.23^a	4.24 ± 0.29^b	1.18 ± 0.03^c	0.399
	90	6.24 ± 0.14^a	3.76 ± 0.09^a	5.22 ± 0.12^a	3.46 ± 0.05^b	1.49 ± 0.07^c	0.432
Propionic acid (PA)	1	0.01 ± 0.00^b	0.05 ± 0.00^c	0.04 ± 0.01^b	0.06 ± 0.01^c	0.06 ± 0.01^c	0.005
	7	0.04 ± 0.00^b	0.06 ± 0.00^c	0.07 ± 0.01^b	0.08 ± 0.02^c	0.08 ± 0.00^c	0.011
	30	0.08 ± 0.01^ab	0.26 ± 0.05^ab	0.24 ± 0.09^a	0.18 ± 0.05^b	0.25 ± 0.05^b	0.021
	60	0.11 ± 0.01^a	0.22 ± 0.05^b	0.23 ± 0.05^a	0.21 ± 0.04^b	0.24 ± 0.05^b	0.014
	90	0.09 ± 0.01^ab	0.31 ± 0.04^a	0.27 ± 0.09^a	0.29 ± 0.09^a	0.38 ± 0.09^a	0.027

SEM = standard error of the mean. Averages followed by different letters on the column do not differ among themselves by Tukey’s test (p < 0.05).