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. 2020 Apr 22;11:1934. doi: 10.1038/s41467-020-15784-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Reconstruction results for microtubules in living cells. A representative time point is shown. The missing structures from U-Net-SIM15 were recovered by scU-Net (white arrows). First panel shows the average projections of 15 SIM raw images; second panel shows the SIM reconstruction; third panel shows the U-Net-SIM15 output; fourth panel shows the scU-Net output. n = 3, from three independent experiments. b Enlarged views of areas indicated by the white-dashed box in a are shown. The dynamics of a single microtubule (white triangle) was well restored by scU-Net. c scU-Net reveals the dynamics of microtubule–mitochondria interactions. First row: average projections of 15 SIM raw images; second row: SIM reconstruction; third row: scU-Net output. n = 3, from three independent experiments. Scale bar: 1 μm.