Table 1.
Summarized thermodynamic data extracted from DSC thermograms thermograms
| Liposome conditions | Tm (°C) major | Tm (°C) minor | Hcal, mcal (Joules) x 10−4 total | Cp (mcal/°C) total | |
|---|---|---|---|---|---|
| Fig. 1 | Panel A | 41.1 | – | 10.65 (0.46) | 2.86 |
| Panel B | 40.8 | – | 24.82 (1.05) | 6.70 | |
| Panel Ca | 40.8 | 38.9 | 53.44 (2.22) | 12.03 | |
| Panel Da | 40.1 | 38.8 | 105.67 (4.19) | 18.39 | |
| Fig. 2 | Panel Aa | 40.5 | 38.1 | 10.61 (0.44) | 2.21 |
| Panel Ba | 40.4 | 38.5 | 23.91 (1.01) | 4.45 | |
| Fig. 3 | Panel Aa | 41.0 | 38.9 | 104.67 (4.38) | 18.99 |
| Panel Ba | 40.1 | 38.8 | 105.67 (4.42) | 18.39 | |
| Panel Ca | 41.6 (40.5) | 38.9 | 117.26 (4.91) | 18.21 | |
| Panel Da | 41.4 | 38.5 | 122.43 (5.13) | 18.81 | |
| Panel Ea | 41.6 | 38.4 | 114.58 (4.80) | 23.70 |
a
In all cases a small peak at approximately 27.0 °C is readily detected and can be attributed to trace amounts of unincorporated lipid. The minor peak has also been previously reported for DPPC liposomes and is referred to as a “pre-transition” phase immediately preceding the main phase transition at approx. 41.0 °C [31]. All Tm values represent the mid-point of the melting temperature range for each transition.