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. 2020 Mar 9;24(8):4612–4623. doi: 10.1111/jcmm.15123

Figure 4.

Figure 4

The effects of different glucose culture times on PHLPP1 expression. A, Western blot analysis of the expression of PHLPP1 in primary neonatal cardiomyocytes. B, Western blot analysis of the expression of PHLPP1 in H9c2 cells. C, Immunofluorescence of PHLPP1 in primary neonatal cardiomyocytes and H9c2 cells. PHLPP1 stained green in primary neonatal cardiomyocytes and red in H9c2 cells; nuclei stained blue with DAPI. All experiments were performed at least 3 times.NG: 5.5 mmol/L glucose, HG: 33.3 mmol/L glucose. Data are expressed as the means ± SD. Statistical analysis was performed using one‐way ANOVA followed by Dunnett's multiple‐to‐one comparison test. *P < .05 compared with NG