Figure 8.

TUNEL analyses and Western blotting demonstrated that LY294002 effectively reversed the anti‐apoptotic effect of PHLPP1 inhibition. A, The cell apoptosis rates detected by TUNEL were reduced after PHLPP1 inhibition; however, this reduction was reversed after pretreated with LY294002. B, The expression of cleaved caspase3 was reduced after PHLPP1 inhibition, while LY294002 reversed this reduction. C, The ratio of Bax to Bcl2 was reduced after PHLPP1 inhibition but it was enhanced when treatment with LY294002. NG: 5.5 mmol/L glucose; HG: 33.3 mmol/L glucose. siN.C: negative control siRNA. siPHLPP1: PHLPP1 siRNA. All experiments were performed at least 3 times. Data are expressed as the means ± SD. Statistical analysis was performed using one‐way ANOVA followed by Bonferroni's post hoc test. *P < .05 compared with HG + siNG. #P < .05 compared with HG + siPHLPP1