Figure 2.

AGAP2‐AS1 affects cell proliferation and apoptosis in trophoblasts. A and B, MTT assays and (C and D) BrdU assays were conducted to test cell viability after AGAP2‐AS1 siRNAs or AGAP2‐AS1 plasmid transfected trophoblasts cells, including HTR‐8/SVneo and JEG3. E and F, Cell cycle analyses by Flow cytometry in HTR‐8/SVneo and JEG‐3 cells after transfected with AGAP2‐AS1 siRNAs or plasmid. G, Cell apoptosis analysis by Flow cytometry after transfected with AGAP2‐AS1 siRNAs in HTR‐8/SVneo. LR, early apoptotic cells; UR, terminal apoptotic cells. H, Cleaved caspase 3 protein analysis after treated with AGAP2‐AS1 siRNAs at 48 post‐transfection by Flow cytometry assays. Numbers are presented as the mean ± SEM, **P < .01, *P < .05