Figure 4.

AGAP2‐AS1 can be activated by transcript factor FOXP1. A and B, FOXP1 expression level at protein and RNA level by Western blotting and qPCR, respectively, when HTR‐8/SVneo cells were transfected with FOXP1‐specific siRNAs. The AGAP2‐AS1 expression level was tested by qPCR after treated with siRNAs against AGAP2‐AS1 (B, right panels). C and D, FOXP1 expression level at protein and RNA level by Western blotting and qPCR, respectively, when HTR‐8/SVneo cells were transfected with FOXP1 plasmid. The AGAP2‐AS1 expression level was tested by qPCR after treated with plasmid (D, right panels). E, ChIP‐qPCR of FOXP1 bind to the promoter of AGAP2‐AS1 in HTR‐8/SVneo, IgG as negative control. **P < .01, *P < .05