Figure 4.

HNG stabilizes platelet microtubules from tubulin deacetylation. (A‐B) Resting platelets or microtubule depolymerization reagent, nocodazole‐treated platelets after incubation with 10 μM HNG, scramble‐HNG or vehicle at 37°C for 10 minutes were centrifugated on poly‐L‐lysine‐coated coverslips. Alternatively, platelets were incubated with HNG and then allowed to adhere to fibrinogen‐coated slides for 1 h at 37℃ before fixation. Samples were stained with β1‐tubulin (A, B) and acetylated tubulin (C, D) antibodies. Representative images are shown. Microtubule stability was analysed using the ratio of ring structure (A, B). *P < .05, ***P < .001, ordinary one‐way ANOVA, Dunnett's multiple comparisons test