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HNG inhibits AKT and ERK phosphorylation downstream of HDAC6. Gel‐filtered human platelets were pre‐incubated with 10 μM HNG for 10 minutes at 37℃ and then stimulated with 2 μg/mL collagen at different time‐points under stirring. After lysis with RIPA buffer, samples were analysed by Western blot, and ERK1/2 (A), AKT (B), PLCgamma2 (C) and P38 (D) phosphorylation was detected using antibodies and quantified. *P < .05, **P < .01, t test
