Figure 2.

CD137 signalling regulated Th17 cells through modulating IL‐6 of ECs‐derived exosomes in vitro. A, IL‐6, IL‐12, IL‐23 and IL‐1β mRNA levels in ECs were measured with qRT‐PCR analysis (*P < .05, n = 3). B, IL‐6 concentration in supernatant and exosomes was measured with ELISA analysis (*P < .05, n = 5). C, ECs were transfected with siRNA IL‐6 or siRNA NC. Then, exosomes were isolated from transfected ECs and incubated with Th17 cell differentiation system. D, Flow cytometric analysis of Th17 cells (*P < .05, n = 5)