Figure 4.

Effect of EVs isolated from primary NPCE cells and NPCE cell line on β‐catenin and GSK3β protein expression in primary TM cells. Primary TM cells were treated with NPCE primary or cell line‐derived EVs and the effect of EVs on protein level of β‐catenin and pGSK3β in TM cells was detected by Western blotting at the indicated time‐points. A, Representative Western blots showing the levels of β‐catenin or (C) pGSK3β protein, phosphorylated at Ser9 in primary TM cells following EV treatment. B, The graph shows the densitometry quantification for the expression of β‐catenin or (D) the expression of pGSK3β in primary TM cells. Data presented as mean ± SEM of three independent experiments. Asterisks indicate statistically significant differences from untreated control (*P < .05, **P < .01 in two‐way ANOVA with Bonferroni's test)