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. 2020 Apr 16;11:679. doi: 10.3389/fmicb.2020.00679

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Copyright © 2020 Dubey, Vélëz, Broberg, Jensen and Karlsson.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In vivo bioassay to test the biocontrol ability of C. rosea strains against B. cinerea and F. graminearum. (A) Measurement of B. cinerea necrotic lesions on detached leaves of A. thaliana plants. The leaves were inoculated with C. rosea strains 60 min before application of B. cinerea and allowed to interact for 60 h. Only pathogen inoculated leaves were used as control. Necrotic lesion area was measured under the microscope using DeltaPix camera and software. The exprement was performed in five biological replicates with six leaves in each replicate in each treatment. (B) Foot rot disease on wheat using sand seedling test. Seedlings were harvested 3 weeks post inoculation and disease symptoms were scored on 0–4 scale. The experiment was performed in five biological replicates with 15 plants in each replicate. Different letters indicate statistically significant differences (P ≤ 0.05) within experiments based on the Fisher’s exact test.