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. 2020 Apr 16;8:232. doi: 10.3389/fcell.2020.00232

FIGURE 1.

FIGURE 1

High expression of ANXA6 predicts radioresistance of NPC. (A) Survival fractions of HNE2, CNE1, and CNE1R cells after irradiation. (B) Volcano plot of differentially expressed genes between CNE1R and CNE1 cells. (C) Volcano plot of differentially expressed genes in the tumor tissue of NPC radioresistant patients (n = 3) before and after radiotherapy. (D) Heat map of the expression levels of 29 differential genes between the above volcano plots, analyzed by Heml (software for drawing volcano maps). (E) ANXA6 mRNA expression levels in HNE2, CNE1, and CNE1R cells. (F) Western blot assay of ANXA6 protein in HNE2, CNE1, and CNE1R cells. (G) Efficiency of siANXA6 transfection in CNE1 and CNE1R cells. (H) Dose responses of survival factions of CNE1 and CNE1R cells after siANXA6 transfection. * P < 0.05 between indicated groups.