Table 1.
Experimental schedule and group assignment.
| Group assignation | Animal number | Injections and dose |
Time of sacrifice/ tissue collection |
|
|---|---|---|---|---|
| Figure 1A | Control | 15 | LPS: 30 mg/kg, once, intraperitoneally Mel: 20 mg/kg/day, for 7 days before LPS treatment, intraperitoneally |
- |
| LPS | ||||
| LPS+Mel | ||||
| Figures 1–3 | Con | 20 | LPS: 6 mg/kg, once, intraperitoneally Mel: 20 mg/kg/day, for 7 days before LPS treatment, intraperitoneally |
6 h after LPS treatment |
| Mle | ||||
| LPS | ||||
| LPS+Mel | ||||
| Figures 4–6 | LPS | 20 | LPS: 6 mg/kg, once, intraperitoneally Mel: 20 mg/kg/day, for 7 days before LPS treatment, intraperitoneally Compound C: 20 mg/kg, once, 30 min prior to the LPS injection |
6 h after LPS treatment |
| LPS+Compound C | ||||
| LPS+Mel | ||||
| LPS+Mel+Compound C | ||||
| Figures 7–9 | LPS+Mel | 20 | LPS: 6 mg/kg, once, intraperitoneally Mel: 20 mg/kg/day, for 7 days before LPS treatment, intraperitoneally Compound C: 20 mg/kg, once, 30 min prior to LPS injection, intraperitoneally 3-MA: 10 mg/kg/day, for 7 days before LPS treatment, intraperitoneally Rapamycin: 1.5 mg/kg/day, for 7 days before LPS treatment, intraperitoneally |
6 h after LPS treatment |
| LPS+Mel+3-MA | ||||
| LPS+Compound C | ||||
| LPS+Compound C+Rapa | ||||
| Figure 10 | Control | - | LPS: 5 μg/ml, for 24 h Mel: 100 μmol/l, 24 h before LPS treatment Compound C: 10 μmol/l, 1 h prior to LPS treatment |
24 h after LPS was added |
| LPS | ||||
| LPS+Mel | ||||
| LPS+Mel+Compound C |