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. 2019 Dec 31;52(2):622–633. doi: 10.4143/crt.2019.593

Fig. 4.

Fig. 4.

Knockdown of chemokine (C-X-C motif) ligand 13 (CXCL-13) overcomes 5-fluorouracil (5-Fu) resistance (5-FuR) in colorectal cancer cells. (A) Box-plot showing the CXCL-13 expression level in DLD-1 5-FuR cells or HCT116 5-FuR cells, DLD-1 5-FuR or HCT116 5-FuR siCXCL-13 cells (30 pg/mL for siCXCL-13). (B, C) Viability of DLD-1 5-FuR cells or HCT116 5-FuR cells, DLD-1 5-FuR or HCT116 5-FuR siCXCL-13 cells (30 pg/mL for siCXCL-13) and recombinant CXCL-13 (10 ng/mL) reversed the effect of CXCL-13 siRNA on the sensitivity of DLD-1 5-FuR cells or HCT116 5-FuR cells treated with different concentrations of 5-Fu for 3 days after pretreatment with CXCL-13 siRNA. (D) The 5-Fu concentration of 50% inhibition of cell growth (IC50) of six cells in B and C above. Data from three independent experiments were tested in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001 by Student’s t test or one-way ANOVA.