Fig. 5.

5-Fluorouracil (5-Fu) treatment induces chemokine (C-X-C motif) ligand 13 (CXCL-13) secretion in vivo. (A) A simple schematic of the experimental process. (B) Line chart showing the serum CXCL-13 expression level in mice bearing patient-derived tumor xenograft (PDX) tumors and treated with vehicle or 5-Fu (30 mg/kg/twice a week for 4 weeks) as in A. (C) Representative H&E, immunohistochemistry (IHC) images of subcutaneous tumors formed by PDX tumors treated as in A. (D) Quantification of IHC staining for CXCL-13 of subcutaneous tumors formed by PDX tumors treated as in A. ELISA, enzyme-linked immunosorbent assay. Data are represented as mean±standard deviation. n=3 mice/group while n=6 tumors/group. **p < 0.01 by Student’s t test or two-way ANOVA.