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. 2020 Apr 16;8:220. doi: 10.3389/fcell.2020.00220

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Copyright © 2020 Azzarelli.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Timeline of in vitro method to culture neural stem cells and glioblastoma cells in 2D and 3D. Schematic representation of the development of different protocols to culture NSCs (neural stem cells) and GBM (glioblastoma) cells in monolayer, spheres and organoids. SFM, serum free medium; EGF, epidermal growth factor; FGF2, fibroblast growth factor 2; GFs, growth factors; NeoCOR, neoplastic cerebral organoids; GLICO, GLIoma cerebral organoids.