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. 2020 Apr 16;11:425. doi: 10.3389/fphar.2020.00425

Figure 4.

Figure 4

N-butanol extract of Taohe-Chengqi decoction (NE-THCQ) reversed epithelial-mesenchymal transformation (EMT) and inhibited extracellular matrix (ECM) accumulation and inflammation in TGF-β1-stimulated HK-2 cells. (A) NE-THCQ treatment cytotoxicity in HK-2 cells. The cytotoxicity of NE THCQ was determined by CCK-8 assay. (B) Effect of NE-THCQ on the expressions of FN, E-cadherin, and a-SMA. (C) The representative images and statistical graph of immunofluorescence staining for FN, E-cadherin, and α-SMA (400 ×). Bar = 20 μm. (D) The representative images and statistical graph of immunofluorescence staining for tumor necrosis factor-α (TNF-α) (400 ×). Bar = 20 μm. Data were presented as mean standard deviation (SD) of three independent experiments. *P < 0.05, **P < 0.01.