Figure 5.

Differential Gene Expression by qPCR Analysis following Gene Delivery in TC2R

Following gene delivery of TC2R cells with either control (pMCS), pIL-27.ns, or pIL-27.pepL, and qPCR analysis, the cells transfected with pIL-27.ns or pIL-27.pepL had different patterns of up-(red) and downregulation (blue) of gene expression relative to control. Fold changes in expression relative to control pMCS are shown at 24 h-post transfection in (A) genes delivered (IL-27p28 and EBI3), (B) IL-6 and IL-27 responsive or target genes, (C) genes representing cytokines in the tumor microenvironment, and (D) immunogenic genes. ∗p < 0.05 relative to control pMCS transfected cells; #p < 0.05 relative to pIL-27.ns transfected cells. Data is expressed as mean +- the standard deviation.