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. 2020 Mar 30;17:717–730. doi: 10.1016/j.omtm.2020.03.025

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© 2020 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Second Downstream Scale-up Run with Lentiviral Vectors Expressing Thymidine Kinase (LV-TK) with Optimized Parameters

(A) Functional titers (TU/mL ±SD) and vector quality in terms of total vps compared to functional transducing units (TUs), as well as TU recoveries from harvest for downstream processing. (B) Total particle concentration vp/mL and vp recovery from harvest for downstream processing. (C) Total protein and dsDNA concentrations (±SD) and percentage clearances for downstream processing. (D) Comparison of dsDNA clearance in the two scale-up runs measured as the concentrations of dsDNA (±SD) during downstream processing. (E) The chromatogram of the second scale-up run showing the absorbance profile (UV280 and UV254) and the elution buffer concentration percentage. (F) Chromatography functional particle titers (TU/mL) and total particle concentration vp/mL in the fractions collected. Functional titer was determined with qPCR. The total vps were calculated from p24 ELISA results.