Table 3.
Main Parameters Studied to Optimize the Chromatographic Capture Step
| Parameter | Conditions Tested | Chromatographic Step |
|---|---|---|
| Starting material for capture | TFF1a stock | AEXb |
| clarified stock | ||
| fresh and frozen stocks | ||
| Additives | sucrose 10% | AEX |
| Flow rate | 1 to 5 CV/minc | AEX |
| Conductivity | diluted (150 mM NaCl) and standard (300 mM NaCl) TFF1 stocks | AEX |
| Type of salt | 0.9 M NaCl and 1 M (NH4)2SO4 | HICd |
a
Concentration and buffer change after clarification using tangential flow filtration.
b
Anion exchange chromatography.
c
Column volumes per minute.
d
Hydrophobic intercation chromatography.