Figure 1.

The siRNA RSH049252-4 Effectively Downregulated the CRMP4 Expression Both In Vitro and In Vivo

(A) Upper: immunoblot analysis of protein expression of CRMP4 in NSCs treated by different siRNA sequences. Lower: quantification of relative protein levels determined from immunoblots. (B) Relative mRNA expression of CRMP4 in the E21 spinal cord of fetuses treated by intra-amniotic phosphate-buffered saline (PBS, null), Ad-RFP, CRMP4 siRNA, BMSC, or CRMP4 siRNA + BMSC injection. (C) Upper: immunoblot analysis of protein expression of CRMP4 in the spinal cord of fetuses treated by intra-amniotic PBS (null), Ad-RFP, CRMP4 siRNA, BMSC, or CRMP4 siRNA + BMSC injection. Lower: quantification of immunoblot result. Relative density of CRMP4 in different groups. siRNA, small interfering RNA. Data are presented as the mean ± SEM. Statistical significance according to a one-way ANOVA with Bonferroni’s multiple comparison test is indicated. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.49053751752599004905375175259900