Table 2.
Primers Used in the Study
| Genes | Accession No. | Primer Sequences (5′→3′)/Exon Location (nt) | Annealing Temperature (°C) | PCR Product (bp) |
|---|---|---|---|---|
| Crmp4 | NM_012934.1 | sense: CCCAGGAGCAGGCACGAAT/696–715 | 60 | 109 |
| antisense: ACGGTGATGGCACGGAACA/804–785 | ||||
| Casepase3 | NM_012922.2 | sense: GGAACGAACGGACCTGTGG/441–459 | 60 | 220 |
| antisense: CGGGTGCGGTAGAGTAAGC/660–642 | ||||
| Caspase9 | NM_031632.1 | sense: GCTGGACGCAGTGTCAAGTTT/1147–1168 | 60 | 185 |
| antisense: CATTGGCGACCCTGAGAAGGAGG/1331–1309 | ||||
| Bcl2 | NM_016993.1 | sense: GAGCGTCAACAGGGAGATG/705–723 | 60 | 170 |
| antisense: GAGACAGCCAGGAGAAATCA/874–855 | ||||
| Bax | NM_017059.2 | sense: TGGAAGAAGATGGGCTGAGGC/651–671 | 60 | 139 |
| antisense: CATTCCCACCCCTCCCAATAAT/789–768 | ||||
| Nf | NM_031073.3 | sense: CGTCCCTGGAAATAGTCATA/255–274 | 60 | 110 |
| antisense: CACGGAGATAAGCAAGAAAT/364–345 | ||||
| Map2 | NM_013066.1 | sense: TTGGGCAGTGATTACTACGAG/1852–1872 | 60 | 152 |
| antisense: GTGCTGTACCCTGCTTCGTGT/2003–1983 | ||||
| Tau | NM_017212.2 | sense: TGGCTCCTTGGATAACATCACC/1005–1026 | 60 | 239 |
| antisense: CGGACACTTCATCGGCTAACG/1243–1223 | ||||
| Synapsin1 | NM_019133.2 | sense: TGGGCAAGGTCAAGGTAGA/928–946 | 60 | 121 |
| antisense: TGGACACGCACATCGTATTTA/1048–1028 | ||||
| Synaptotagmin | NM_001033680.2 | sense: CGCTGAGAAAGAAGAGCAAGA/1134–1154 | 60 | 131 |
| antisense: ATAAGCCACCCACATCCATC/1264–1245 | ||||
| Tubulin | NM_13924. 2 | sense: CAAGCGCATCTCCGAGCAGTTTAC/1183–1206 | 60 | 196 |
| antisense: CTCCTCGTCGTCATCTTCATACATC/1378–1354 | ||||
| Hb9 | NM_001271274.1 | sense: TCCAGAACCGCCGAATGAAATG/903–924 | 60 | 136 |
| antisense: AGCAGCTCCTCCTCCGTCTTCTCC/1038–1051 | ||||
| Brn3a | sense: ATCGCGGTGTCCCAGGGCAAGA/495–516 | 60 | 168 | |
| antisense: CGAGATGTGGTCCAGCAGGTCA/662–641 | ||||
| Ngf | NM_001277055.1 | sense: GCGTAATGTCCATGTTGTTC/178–197 | 58 | 123 |
| antisense: GTTTAGTCCAGTGGGCTTCA/300–281 | ||||
| Egf | NM_012842.1 | sense: CCACGGTTACATTCACTCC/1063–1081 | 60 | 144 |
| antisense: GCTATCCAAATCGCCTTC/1206–1189 | ||||
| Igf | NM_001082479.1 | sense: ATGGGGAAAATCAGCAGTC/281–299 | 60 | 130 |
| antisense: TGGTAAAGGTGAGCAAGCA/410–392 | ||||
| Fgf2 | NM_019305.2 | sense: CACGTCAAACTACAGCTCCAA/704–724 | 60 | 152 |
| antisense: GACTCCAGGCGTTCAAAGA/855–837 | ||||
| Fgf10 | NM_012951.1 | sense: GTTGTTGCCGTCAAAGCCATTA/379–400 | 60 | 163 |
| antisense: TGCCGTTGTGCTGCCAGTT/541–523 | ||||
| BDNF | NM_012513.4 | sense: GTCACAGTCCTGGAGAAAGTC/1156–1176 | 60 | 149 |
| antisense: GATTGGGTAGTTCGGCATT/1304–1286 | ||||
| Tgf-beta | NM_021578.2 | sense: TCAAGTCAACTGTGGAGCAA/624–643 | 60 | 108 |
| antisense: GCCACTCAGGCGTATCAG/731–714 | ||||
| P75 | NM_012610.2 | sense: CTGGGCTGATGCTGAATGCG/662–681 | 60 | 216 |
| antisense: ACAGGAATGAGGTTGTCGGTGGT/855–877 | ||||
| Vegf | NM_001110335.1 | sense: GCAGCATAGCAGATGTGAA/435–453 | 60 | 129 |
| antisense: TGAACGCTCCAGGATTTA/563-546 | ||||
| Nt3 | NM_031073.3 | sense: CGTCCCTGGAAATAGTCATA/255–274 | 60 | 110 |
| antisense: CACGGAGATAAGCAAGAAAT/364–345 | ||||
| GAPDH | NM_017008.4 | sense: TGCCGCCTGGAGAAACCTGC/808-827 | 60 | 168 |
| antisense:AGCAATGCCAGCCCCAGCAT/956–975 |