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. 2020 Mar 31;21(7):2429. doi: 10.3390/ijms21072429

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Immunolocalization of adherens junction proteins E-cadherin and β-catenin. MCF-7 and 6D cells treated or not treated with 10 µM CBD were cultured for 48 h, fixed, and stained with specific antibodies to E-cadherin and β-catenin. (A) E-cadherin is localized in the periphery of MCF-7 cells. Adherens junctions are indicated by arrows. (B) β-catenin in MCF-7 cells also is localized at the intercellular junctions (arrows). (C) In 6D cells stained to visualize E-cadherin the protein is in the cytoplasm and around the nuclei (arrows). (D) β-catenin in the dispersed 6D cells is localized in the nuclei (arrows) and a faint signal is still visible in the remaining junctions (empty arrow). (E) 6D cells treated with 10 µM CBD showed E-cadherin normal localization in the periphery of the cells making contact (arrows). (F) In 6D cells treated with CBD, β-catenin is localized and mostly increased in the reconstituted adherens junctions (arrows). In addition, β-catenin is no longer detected in the nuclei. Bar = 50 µm.