Figure 7.

The CBD decreased the expression and activation of AKT, blocking cell migration. (A) Representative Western blot of AKT in MCF-7 and 6D treated or not treated with CBD and 6D cells treated with 100 µM wortmannin. Densitometric values correspond to total AKT obtained from three independent experiments (n = 3) ± SD. All values were normalized to actin and expressed relative to those in MCF7 cells. Asterisks indicate p < 0.05. (B) Representative Western blot of pAKT(Ser473) in the conditions mentioned above. Percentage of AKT phosphorylation expressed as pAKT/AKT ratio. (C) To illustrate the effect of wortmannin inhibition of AKT phosphorylation in the cell migration, wound healing assays were performed with 6D, CBD-treated and wortmannin-treated cells. The percentage of the wound closure was calculated as done in Figure 2. Asterisks indicate p < 0.05.