Figure 7.

Lung oxidative stress in endothelial ERK2-sufficient (eERK2^+/+) or –deficient (eERK2^+/−) mice exposed to hyperoxia. One-day-old eERK2^+/+ or eERK2^+/− mice were exposed to either 21% O₂ (normoxia) or 70% O₂ (hyperoxia) for one week, following which the lung tissues were harvested for gene expression studies. (A–C) RT-PCR analyses-based determination of NQO1 (A), HO1 (B), and GPX2 (C) mRNA levels. (D) Determination of SOD1 and SOD2 protein levels by immunoblotting. (E,F) Quantification and normalization of SOD1 (E) and SOD2 (F) band intensities to those of β-actin. Values are presented as mean ± SD (n = 5–6/genotype/exposure). Significant differences between normoxia and hyperoxia groups are indicated by *, p < 0.05 (ANOVA).