Table 3.

Summary of release criteria for pluripotency markers detected by immunocytochemistry used for the establishment of hPSC lines according to cGMP.

hPSCs	Oct3/4	SSEA3	SSEA4	TRA-1-60	TRA-1-81	Sox2	Nanog	AP (TRA-2-54)	SSEA1	Source
hESCs	≥60%*	≥60%*	≥60%*	≥60%*	≥60%*	-	≥60%*	-	-	Tannenbaum [33]
hESCs	≥70%	-	≥70%	≥70%	≥70%	≥70%	≥70%	-	NA	Ye [43]

NA—no release criterium available, AP—alkaline phosphatase; *colonies; - not tested.