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. 2020 Apr 1;21(7):2446. doi: 10.3390/ijms21072446

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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SCD was significantly up-regulated in differentiated porcine adipocytes. Porcine preadipocytes were primarily cultured and differentiated into mature adipocytes in vitro. (A) Left: nondifferentiated preadipocytes; right: oil red O staining of the differentiated cells. Positive staining indicated lipid droplet formation. Scale bar, 100 μm. (B) The expression levels of SCD and the adipocyte differentiation marker genes PPARG and C/EBPA in preadipocytes and differentiated adipocytes. TUBULIN was used as a loading control. (C) The expression levels of genes related to lipid synthesis were measured in preadipocytes and differentiated adipocytes (n = 3/group), * p < 0.05.