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. 2020 Apr 1;21(7):2446. doi: 10.3390/ijms21072446

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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SCD knockout inhibited PEF transdifferentiation into mature adipocytes. (A) SCD^+/+ and SCD^-/- were differentiated into adipocytes in vitro. Cells were subjected to oil red O staining after differentiation. Scale bar, 100 μm. (B) Western blot analysis of SCD in nondifferentiated and differentiated SCD^+/+ and SCD^-/- cells. TUBULIN was used as the loading control. Notably, SCD was undetectable in differentiated SCD^-/- cells. (C) The expression levels of the genes related to lipid synthesis, in both the SCD^+/+ and SCD^-/- cells. Notably, all of these genes were significantly lower in the SCD^-/- cells (n = 3/group). (D,E) TG (n = 7/group) and NEFA (n = 6/group) content levels in the SCD^+/+ and SCD^-/- cells. (F) The expression levels of the genes related to fatty acid β-oxidation in both the SCD^+/+ and SCD^-/- cells (n = 3/group); * p < 0.05, ** p < 0.01 and *** p < 0.001.