Figure 2.

Eact does not activate endogenous Ca²⁺-dependent Cl⁻ secretion, and has little effect on [Ca²⁺]_i. (A,B) No activation of iodide quenching by Eact or GSK1016790 (n = 5 for both). (C) RT-PCR indicating the expression of TRPV4 in HT₂₉ cells. (D,E) No activation of whole cell currents in HT₂₉ cells by Eact or GSK1016790 (10 µM; n = 5 for both). (F,G) RT-PCR of TRPV4 expressed in CFBE (F) and HEK293 (G) cells. (H) ATP (100 µM) induced [Ca²⁺]_i rise is inhibited by knockdown of TMEM16A but not by Ani9 (10 µM; n = 37–105). (I,J) Minor increase in [Ca²⁺]_i by Eact in HT₂₉, CFBE, or HEK293 cells (n = 31–111). Mean ± SEM. ^# significant inhibition (p < 0.05; unpaired t-test).