Figure 8.

TMEM16F does not contribute to ion transport in mouse airways. Original Ussing chamber recordings obtained under open circuit conditions in tracheas from TMEM16F^fl/fl (wild type) (A,C) and TMEM16F^fl/flFoxJ1Cre (TMEM16F knockout in ciliated airway epithelial cells) (B,D) mice. Inhibition of epithelial Na⁺ absorption (via ENaC) by amiloride (10 µM), activation of CFTR by IBMX and forskolin (100 µM and 2 µM; cAMP), and activation of Ca²⁺-dependent Cl⁻ secretion by luminal ATP (100 µM) and basolateral carbachol (CCH; 100 µM) were comparable in wild type and TMEM16F-knockout tracheas. (E) Summaries of the calculated short circuit currents. Mean ± SEM (4 tracheas for each series).