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. 2020 Apr 2;21(7):2476. doi: 10.3390/ijms21072476

Figure 1.

Figure 1

Quantification of the number of MSCs, detected as CFU-Fs, and those MSCs with native osteogenic potential (CFU-O) within whole marrow aspirates from AMC and T2DM cohorts. (A) Bone marrow from donors living with T2DM (n = 39) contained reduced numbers of CFU-Fs compared to bone marrow from the AMC cohort (n = 12). These data were normalised to MNC number and indicate the number of MSCs in the bone marrow is reduced in response to the T2DM environment. (B) CFU-O numbers in fresh bone marrow were identified by alkaline phosphatase staining and normalised to MNC number as per CFU-F. A reduction in the number of CFU-Os was recorded in the bone marrow in the T2DM cohort (n = 9) compared to that from the AMC cohort (n = 22). (C) In evaluating CFU-O number as a proportion of all CFUs in a donor bone marrow by two-way factorial analysis of deviance, diabetic status was observed to lead to a reduction in the proportion of CFU-Os. All data are displayed as mean ± SEM. * = p ≤ 0.05; ** = p ≤ 0.01.