Figure 5.

MSC mobility is not impacted by the diabetic status of the marrow donor. (A) As assayed through a transwell culture system, the (i) chemokinetic and (ii) chemotactic migration of MSCs from AMC (n = 6) and T2DM (n = 3) cohorts are comparable. (B) Representative images demonstrate the underside of a transwell with the migrated nuclei stained with Hoechst. (C) Chemokine levels in conditioned media at higher concentrations (i) and at lower concentrations (ii). Multiple T-test in R demonstrated no significant difference in levels (n = 4 per cohort). All data are displayed as mean + SEM, ns = not significant, i.e., p > 0.05.