Figure 1.

Expression profile analysis of OsNAC006. (A) Detection of OsNAC006 expression in various tissues and organs of rice using RT-qPCR. Four-week-old seedlings were used to harvest root, sheath and leaf samples at the seedling stage. Plants in stages before the heading stage were used to harvest root, stem, sheath, leaf and panicle samples at the reproductive growth stage. Error bars indicate the standard error (SE) based on three biological replicates. (B), Nuclear localization of OsNAC006 protein in the rice protoplast. NLS, Nuclear localization signal. Scale bar = 20 µm. (C) Expression levels of OsNAC006 under various abiotic stresses and hormone treatments. Four-week-old seedlings were subjected to treatment with cold (4 °C), heat (42 °C), PEG 6000 (20%, w/v), NaCl (200 mm), H₂O₂ (1%), IAA (100 μm), ABA (100 μm) and GA3 (100 μm). The relative expression level of OsNAC006 was measured by RT-qPCR at the indicated times. Error bars indicate SE based on three independent biological replicates.