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Using the CRISPR-Cas9 system to create mutants. (A) Design of sgRNA sites for OsNAC006 exons. (B) Single-strand conformation polymorphism analysis of 11 independent OsNAC006-sgRNA01 T0 lines. M, Markers; WT, Wild-type. (C) Sanger sequencing of the target site in OsNAC006-sgRNA01 T0 lines. (D) Phenotypic analysis of OsNAC006 T0 mutant lines.
