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. 2020 Apr 10;21(7):2643. doi: 10.3390/ijms21072643

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Mass spectroscopy lipidomic analysis of whole cells and plasma membrane preparations. (A) Total phospholipids; (B) Short, long and very long glycerophospholipids; (C) Total cholesterol; (D) Phospholipid saturation level (DB = double bond); (E) Phosphatidylcholine distribution according to chain length; (F) Phosphatidylethanolamine distribution according to chain length. Bras: solid black, whole cells treated for GPMV preparation; striped, GPMV (blebs); empty, cells treated for PM patch preparation; dotted PM patches. Significance: (*) p < 0.0 (**); p < 0.01; (***) p < 0.001.